Effects of intranasal interferon gamma on transforming growth factor-β1/Smad in rats with allergic rhinitis
10.3760/cma.j.issn.1673-0860.2011.01.021
- VernacularTitle:鼻腔吸入γ干扰素对大鼠变应性鼻炎转化生长因子β1/Smad信号通路的影响
- Author:
Qin LI
1
;
Ping KONG
;
Cai-Xia WU
;
Chong-Wei SUN
;
Yan-Lin CHEN
;
Xiao-Qun XU
;
Da-Liang ZHANG
Author Information
1. 山东省临沂市人民医院
- Keywords:
Disease models,animal;
Rhinitis,allergic,perennial;
Interferon type Ⅱ;
Rats;
Transforming growth factor beta 1;
Smad proteins
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2011;46(1):59-62
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of intranasal interferon gamma (IFN-γ) on nasal mucosa remodeling and expression of transforming growth factor-β1 ( TGF-β1 ), Smad2, Smad3, Smad7 in allergic rhinitis (AR) rat model. Methods Ovalbumin (OVA) and aluminum hydroxide were used to construct the AR model. Thirty AR rats were randomly divided into positive control group (group B, n =10), IFN-γ treatment group( group C, n = 10) and negative control group( normal rats, n = 10). After the AR models were built, 50μl PBS, 1 μg IFN-γ was dropped into the nasal cavity of each rat in group B and group C, from the fouth week to tenth week, twice a week. The nasal mucosa was collected on day 71 in order to observe the pathologic changes, and the expression of TGF-β1, TGF-β1 mRNA, Smad2 mRNA,Smad3 mRNA and Smad7 mRNA by immunohistochemistry and reverse transcriptase-polymerase chain reaction. Results Decreases of TGF-β1, Smad2 and Smad3 mRNA were seen in nasal tissue of group C (0. 59 ±0. 04, 0. 39 ±0. 08, 0. 46 ±0. 15) as compared with group B (0. 82 ±0. 12, 0. 70 ±0. 18, 0. 95 ±0. 26) , the differences were significant ( q value were 3.15, 4. 47, 3.03, all P ＜ 0. 05 ). The levels of Smad7 mRNA expression increased significantly ( q = 2.98, P ＜ 0. 05 ) in group C ( 0. 31 ± 0. 05 ) as compared with group B ( 0.25±0.06). Immunohistochemistry showed significant decrease of TGF-β1expression in the nasal tissue of group C much lesser than that in group B. Conclusions Intranasal IFN-γcould decrease the expression of TGF-β1, TGF-β1 mRNA, Smad2 mRNA, Smad3 mRNA, increase the expression of Smad7 mRNA in AR rats model and inhibit the nasal mucosa remodeling.
