Expression of fragile histidine triad (FHIT) protein and its significance in diagnosing classical Hodgkin lymphoma.
- Author:
Po ZHAO
1
;
Ya-li LÜ
;
Mei ZHONG
;
Ling-hong CHEN
;
Xiao-lu PU
Author Information
- Publication Type:Journal Article
- MeSH: AC133 Antigen; Acid Anhydride Hydrolases; metabolism; Adolescent; Adult; Aged; Aged, 80 and over; Antigens, CD; metabolism; Antigens, CD20; metabolism; Biomarkers, Tumor; metabolism; Cell Nucleus; metabolism; Child; Child, Preschool; Cytoplasm; metabolism; Female; Glycoproteins; metabolism; Hodgkin Disease; diagnosis; metabolism; Humans; Immunohistochemistry; Male; Middle Aged; Neoplasm Proteins; metabolism; Peptides; metabolism; Reed-Sternberg Cells; metabolism; Sensitivity and Specificity
- From: Chinese Journal of Pathology 2006;35(5):289-291
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the expression of FHIT protein and its potential application in diagnosing classic Hodgkin lymphoma.
METHODSImmunohistochemical study using EnVision method for FHIT tumor suppressor protein, hematopoietic stem cell markers CD133/AC133 and CD34, B-cell marker CD20, T-cell marker CD3 and oncoprotein c-erbB2 was performed on 33 cases of classic Hodgkin lymphoma.
RESULTSThirty-three of the Hodgkin lymphoma cases (90.9%) expressed FHIT protein. The antigen was mainly located in the cytoplasm, nucleus and membrane of classic Reed-Sternberg and Reed-Sternberg-like cells. Normal B and T lymphocytes, as well as their malignant counterparts, were negative for FHIT protein; whereas monocytes, histiocytes and dendritic cells were positive. All the cases studied were negative for CD133/AC133, CD34, CD3 and c-erbB-2. Two of the 33 cases showed positive staining for CD20 in some of the Reed-Sternberg cells.
CONCLUSIONThe expression of FHIT protein can be used as a useful adjunct in diagnosing classic Hodgkin lymphoma.
