Inhibition of the expression of p42MAPK in HeLa cell line by RNA interference.

Author: Chen HUANG ¹ ; Li-ying LIU ; Tu-sheng SONG ; Lei NI ; Li-ping SONG ; Jing-song HU ; Xiao-ge ZHAO ; Lü-sheng SI
Author Information

1. Key Laboratory of Environment and Gene Related to Diseases, Ministry of Education, Medical School of Xi'an Jiaotong University, Xi'an 710061, China.
Publication Type:Journal Article
MeSH: Cell Cycle; Cell Proliferation; Cell Survival; Flow Cytometry; HeLa Cells; Humans; Mitogen-Activated Protein Kinase 1; biosynthesis; genetics; RNA Interference; RNA, Small Interfering; genetics; Transfection
From: Chinese Journal of Pathology 2006;35(5):292-295
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo screen for siRNAs that inhibit the expression of p42(MAPK) in HeLa cell line.
METHODSThree p42(MAPK) siRNAs and one random siRNA were synthesized using Silencer siRNA Construction Kit, and labeled with Cy-3 for measurement of transfection effect. SiRNAs were transfected into HeLa cells by Lipofectamin 2000. The expression of p42(MAPK) was analyzed by Western blot. The biological effect of siRNAs on HeLa cell growth was monitored by MTT and flow cytometry.
RESULTSTwo siRNAs (siRNA-2 and siRNA-3) among three tested were identified to be able to downregulate the p42(MAPK) expression. A concurrent growth retardation of HeLa cell line was observed in comparison with the control.
CONCLUSIONInhibition of p42(MAPK) expression with siRNA technique can inhibit the proliferation of HeLa cells.