A new method for isolating CD34(+) cells based on complex of magnetic nanoparticles and antibody.
- Author:
Li-Na ZHAO
1
;
He-Bai SHEN
;
Wei CHEN
;
Long-Zhang ZHU
Author Information
1. Life and Environment Science College, Shanghai Normal University, Shanghai 200234, China.
- Publication Type:Journal Article
- MeSH:
Antigens, CD34;
metabolism;
Fetal Blood;
cytology;
Flow Cytometry;
Hematopoietic Stem Cells;
cytology;
immunology;
Humans;
Immunomagnetic Separation;
methods;
Magnetics;
Nanoparticles
- From:
Journal of Experimental Hematology
2007;15(3):537-541
- CountryChina
- Language:Chinese
-
Abstract:
The purpose of this study was to synthesize the complex of magnetic nanoparticles and antibody, and to apply them to isolate the CD34(+) cells from umbilical cord blood, then to evaluate its separation efficiency. The complex of magnetic nanoparticles and antibody was used to separate CD34(+) cells from umbilical cord blood in the outer magnetic field because of its superparamagnetism, specific identification and function of combination with CD34(+) cells. Scanning electron microscopy was used to observe the morphology of the separated CD34(+) cells. Flow Cytometer was applied to evaluate the sorting efficiency of magnetic nanoparticles, liquid culture and colony culture were taken to assay proliferation and differentiation capacity of the separated CD34(+) cells. The results showed that the CD34(+) cells from umbilical cord blood were isolated fast and effectively by the complex of magnetic nanoparticles and monoclonal antibody. Moreover, the isolated CD34(+) cells still maintained its normal morphology, highly proliferative and differentiative capacity. It is concluded that the complex of magnetic nanoparticles and monoclonal antibody has been successfully synthesized and developed as a technique which efficiently and quickly isolates CD34(+) cells from umbilical cord blood.
