Mechanism underlying IL-2 inhibition on T cell specific immune response.
- Author:
Teng-Long ZHANG
1
;
Yan-Hui XIE
;
Ying WANG
;
Guo-Wei LIN
Author Information
1. Department of Hematology, Huashan Hospital, Fudan University, Shanghai 200040, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Gene Expression;
Humans;
Interleukin-2;
immunology;
pharmacology;
Mice;
Suppressor of Cytokine Signaling 3 Protein;
Suppressor of Cytokine Signaling Proteins;
immunology;
metabolism;
T-Lymphocytes;
immunology
- From:
Journal of Experimental Hematology
2007;15(3):573-577
- CountryChina
- Language:Chinese
-
Abstract:
The study was aimed to investigate the effect of IL-2 on the acquired immune response of naive T cells, and to explore the influence of IL-2 on suppressor of cytokine signaling 3 (SOCS-3) expression of naive T cells, as well as to elucidate the role of SOCS-3 on antigen specific immune response in vitro. Naive DO11.10 T cells were pre-stimulated with IL-2 (50 U/ml), and stimulated with OVA(323 - 329) antigen after removing IL-2, then the proliferation of naive DO11.10 T cells was detected. Naive DO11.10 T cells were stimulated with IL-2 (50 U/ml), and SOCS-3 expression was detected by real-time PCR. Naive DO11.10 T cells were stimulated with OVA(323 - 329) antigen, and SOCS-3 expression was detected by means of (3)H-TdR. The results showed that after IL-2 pre-stimulation, the proliferation of naive DO11.10 T cells decreased significantly when stimulated with OVA(323 - 329) antigen; SOCS-3 expression of naive DO11.10 T cells was up-regulated significantly after IL-2 stimulation, the up-regulation began obviously at 4 hours and reached peak at 6 hours. SOCS-3 expression on naive DO11.10 T cells was down-regulated markedly after OVA(323 - 329) antigen stimulation, the expression level of SOCS-3 was lowest on day 2 and returned to normal on day 4 after stimulation. It is concluded that the antigen specific immune response of naive DO11.10 T cells is inhibited after pre-stimulation with IL-2, which may be mediated by SOCS-3.
