Immunophenotyping characteristics of T-cell acute lymphoblastic leukemia.
- Author:
Li-Juan CHEN
1
;
Jian-Yong LI
;
Yu-Jie WU
;
Hui YANG
;
Si-Xuan QIAN
;
Han-Xin WU
;
Hua LU
;
Wei XU
;
Rui-Lan SHENG
Author Information
1. Department of Hematology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province People Hospital, Nanjing 210029, China.
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Aged;
Antigens, CD;
metabolism;
Antigens, CD34;
metabolism;
Antigens, Differentiation, Myelomonocytic;
metabolism;
CD3 Complex;
metabolism;
Child;
Child, Preschool;
Female;
Humans;
Immunophenotyping;
Infant;
Male;
Middle Aged;
Precursor T-Cell Lymphoblastic Leukemia-Lymphoma;
diagnosis;
immunology;
Sialic Acid Binding Ig-like Lectin 3;
Young Adult
- From:
Journal of Experimental Hematology
2007;15(4):692-695
- CountryChina
- Language:Chinese
-
Abstract:
The objective of this study was to investigate the immunophenotypic characteristics of T-cell acute lymphoblastic leukemia (T-ALL). Immunophenotyping was performed in 140 T-ALL patients by flow cytometry using a panel of monoclonal antibodies and CD45/SSC gating. The results showed that the T-lineage-associated antigen expressions were CD7 > CD2 > CD3 > CD5 successively. The positive rate of CD10 was 19.42% in patients. Among 140 cases of T-ALL, 12 (8.57%) was accompanied by B-lineage associated antigen expression. Myeloid antigen expression was identified in 31 out of 136 cases (22.79%). None of them expressed CD14 antigen. The positive rate of CD34 was 31.06%. The positive rate of myeloid antigen expression in CD34(+) T-ALL (36.58%) was significantly higher than that in CD34(-) T-ALL (15.38%) (p < 0.01). The expression of CD3 in child T-ALL was higher than that in adult T-ALL, whereas the expression of CD33 in children was lower than that in adults. It is concluded that immunophenotyping is an important tool for diagnosis of T-ALL. Immunophenotypic characteristics of T-ALL is heterogeneous.
