Synergistic reversal effect of Chinese medicine compound FFJZ combined with cyclosporine A on multidrug resistance of leukemia K562/VCR cell line.
- Author:
Bin LIAO
1
;
Ren-Ying GE
;
Xia CHEN
;
Zhen-Ping HUANGFU
;
Yan QI
;
Yong-Ping SONG
;
Xu-Dong WEI
Author Information
1. Department of Hematology, The People Hospital of Fujian Province, Fujian Traditional Chinese Medical College, Fuzhou 350004, China. liaobin@csco.org.cn
- Publication Type:Journal Article
- MeSH:
Cyclosporine;
pharmacology;
Drug Resistance, Multiple;
drug effects;
Drug Resistance, Neoplasm;
drug effects;
Drug Synergism;
Drugs, Chinese Herbal;
pharmacology;
Humans;
K562 Cells;
Vincristine;
pharmacology
- From:
Journal of Experimental Hematology
2007;15(4):752-755
- CountryChina
- Language:Chinese
-
Abstract:
The study was purposed to investigate the synergistic reversal effect of Chinese medicine compound FFJZ in combination with cyclosporine A (CsA) on the multidrug resistance (MDR) of human leukemia K562/VCR cell line, as to search effective combination of MDR modulators. MTT (methyl-thazol-tetrazolinum) assay were used to determine the cytotoic and reversal effects on K562/VCR cell line, FCM (flow cytometry) was used to assess the intracellular adriamycin (ADM) concentration and the expression of P-gp in cells. The results showed that the FFJZ in combination with CsA could reverse the drug-resistance of K562/VCR cells and increase the sensitivity K562/VCR cells to adriamycin. They had not the toxic effect on the K562/VCR cells in effective dose and no significant influence on P-gp positive rate of the K562/VCR cells. It is concluded that the FFJZ in combination with CsA may become a safe and effective multidrug resistance-reversing agent with low toxicity in leukemia chemotherapy.
