Expression of BMP-4 in stromal cells in vitro derived from human aorta-gonad-mesonephros region.
- Author:
Hui-Qin CHEN
1
;
Xu-Chao ZHANG
;
Yan-Feng WU
;
Bei-Yan WU
;
Shao-Liang HUANG
Author Information
1. Center for Stem Cell Research, The Second Affiliated Hospital, SUN Yat-Sen University, Guangzhou 510120, China.
- Publication Type:Journal Article
- MeSH:
Aorta;
cytology;
embryology;
metabolism;
Bone Morphogenetic Protein 4;
metabolism;
Cells, Cultured;
Gonads;
cytology;
embryology;
metabolism;
Hematopoietic Stem Cells;
cytology;
Humans;
Mesonephros;
cytology;
embryology;
metabolism;
RNA, Messenger;
metabolism;
Stromal Cells;
metabolism
- From:
Journal of Experimental Hematology
2007;15(4):772-775
- CountryChina
- Language:Chinese
-
Abstract:
The objective of this study was to investigate the expression of BMP-4 in stromal cells in vitro derived from human aorta-gonad-mesonephros (AGM) region. Stromal cells derived from human AGM region (hAGM S1-S5) and fibroblasts derived from human fetal trunk (hFT) were cultured in vitro. RT-PCR was used to analyze the expression of BMP-4 in hAGM S1-S5 stromal cells at mRNA level. And BMP-4 level was detected in the supernatant of hAGM S1-S5 stromal cells by ELISA assay. hFT cells were used as control group. The results showed that the heterogenous hAGM S1-S5 stromal cells displyed shapes of fibroblast-like and endothelial-like cells. hAGM S1-S5 stromal cells expressed BMP-4 mRNA, but fetal trunk fibroblasts (hFT) did not express BMP-4 mRNA. In the supernatant of hAGM S1-S5 cells, BMP-4 could be detected by ELISA assay ana its levels were statistically higher than that in hFT group (p < 0.05), while there was no significant difference between groups of hAGM S1-S5 (p > 0.05). It is concluded that human AGM-derived stromal cells in vitro express BMP-4, and the establishment of a new culture system based on the feeder cells of AGM stroma would promote the differention of embryonic stem cells into hematopoietic stem cells at a high proportion.
