Identification and sequence analysis of a null HLA-B allele HLA-B*5408N newly detected.
- Author:
Qin-Feng LÜ
1
;
Fa-Ming ZHU
;
Wei ZHANG
;
Jun-Jun HE
;
Wei WANG
;
Zhe-Dong HAN
;
Li-Xing YAN
Author Information
1. Blood Center of Zhejiang Province, Key Laboratory of Blood Safety Research, Ministry of Health, Hangzhou 310006, China.
- Publication Type:Journal Article
- MeSH:
Alleles;
China;
Exons;
genetics;
HLA-B Antigens;
genetics;
Humans;
Polymerase Chain Reaction;
Sequence Analysis, DNA
- From:
Journal of Experimental Hematology
2007;15(4):870-872
- CountryChina
- Language:Chinese
-
Abstract:
The study was purposed to investigate the molecular genetic basis for HLA novel allele HLA-B*5408N in Chinese population. DNA was extracted from whole blood by commercial DNA extraction kit, the HLA-B exons 2 - 4 of the proband was amplified by allele specific primers PCR and the amplified product was sequenced for exons 2, 3 and 4 bidirectionally. The sequencing results showed HLA-B alleles of the proband as B*1527 and the novel allele. The sequences of the novel allele have been submitted to Genbank (DQ295998, DQ295999, DQ296000). After blast analysis, the novel allele showed a single nucleotide mismatch with HLA-B*5401 in exon 3 at position 553 G-->T, which resulted in an amino acid changing from Glu to premature stop codon at position 161. No the HLA-B54 antigen specificity expression in the proband cells was found using HLA-AB serological Typing Trays. It is concluded that this allele is a novel null allele and has been officially named B*5408N by the WHO Nomenclature Committee.
