Expression of wnt5a gene in hematologic diseases and leukemic cell lines.
- Author:
Zhao-Quan LI
1
;
Wei-Ke SI
;
Jing PAN
;
Yuan YUAN
;
Quan-Ming ZOU
Author Information
1. Department of Clinic Hematology, The Third Military Medical University, PLA, Chongqing 400038, China.
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Aged;
Child;
Down-Regulation;
Gene Expression Regulation, Leukemic;
Hematologic Neoplasms;
genetics;
metabolism;
Humans;
Middle Aged;
Proto-Oncogene Proteins;
metabolism;
Reverse Transcriptase Polymerase Chain Reaction;
Tumor Cells, Cultured;
Wnt Proteins;
metabolism;
Wnt-5a Protein;
Young Adult
- From:
Journal of Experimental Hematology
2007;15(5):927-930
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the expression level of Wnt5a gene in some hematologic diseases and leukemic cell lines so as to provide a basis for further research of Wnt5a role and its mechanism in hematologic malignancies. The mononuclear cells of peripheral blood and bone marrow were isolated by human lymphocytic isolation solution. The expression of Wnt5a gene in specimen of 31 cases and three leukemic cell lines (Jurkat, K562, HL-60) were detected by RT-PCR. The results showed that in four out of five AML cases, negative or weak positive expressions were observed and negative expressions were observed also in K562 and HL-60 cells. Only in one AML case with complete remission and Jurkat cells the strong positive expressions were observed. The negative expression was observed in all six CML cases. In three out of four ALL cases, the expression was positive or weak positive and one negative. The expressions in two CLL cases were negative. Out of two MM cases, the expression in one was weak positive and in other was negative. Out of three lymphoma cases, the expression in one case was weak positive and in other two cases were negative. There were positive or weak positive expressions in two cases of AA, two cases of IDA, three cases of ITP, one cases of PV and ET cases. It is concluded that there have obvious down-regulated or lost expression of Wnt5a gene in 31 cases of hematologic disease and myelocytic leukemic cell lines except ALL samples. Nevertheless there have general positive expression of Wnt5a in cases of non-malignant hematologic diseases. These results suggest that the genesis of myelocytic leukemia is related to the down-regulated expression of Wnt5a.
