Adenovirus-mediated PDCD5 gene transfer sensitizes apoptosis of K562 cells induced by etoposide.
- Author:
Guo-Rui RUAN
1
;
Shan-Shan CHEN
;
Yan CHANG
;
Jin-Lan LI
;
Ya-Zhen QIN
;
Ling-Di LI
;
Le HAO
;
Jia-Yu FU
;
Yan-Rong LIU
;
Xiao-Jun HUANG
Author Information
1. Institute of Hematology and People's Hospital, Peking University, Beijing 100044, China.
- Publication Type:Journal Article
- MeSH:
Adenoviridae;
genetics;
metabolism;
Antineoplastic Agents, Phytogenic;
pharmacology;
Apoptosis;
drug effects;
Apoptosis Regulatory Proteins;
genetics;
metabolism;
Etoposide;
pharmacology;
Humans;
K562 Cells;
Neoplasm Proteins;
genetics;
metabolism;
RNA, Messenger;
metabolism;
Recombinant Proteins;
genetics;
metabolism;
Transfection
- From:
Journal of Experimental Hematology
2007;15(5):936-940
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to investigate the effect of adenovirus-mediated transfer of PDCD5 gene on apoptosis of K562 cells induced by etoposide. Recombinant adenovirus PDCD5 (Ad-PDCD5), control vectors Ad-null and Ad-eGFP were constructed by AdMax vector system respectively. After K562 cells were transfected by Ad-PDCD5, Ad-null or Ad-eGFP with different multiplicity of infection (MOI), the expression level of the PDCD5 gene was examined by RQ-RT-PCR assay. The effects of etoposide in combination with Ad-PDCD5 on the proliferation and apoptosis of K562 cells were measured by using MTT assay and flow cytometry with Annexin-V-FITC/PI dual labeling technique, respectively. The results showed that the transfection efficiencies of Ad-eGFP in K562, Jurkat and CEM cells ranged from 60% to 86%. Expression level of PDCD5 gene in K562 cells was evidently increased following transfection with Ad-PDCD5. The Ad-PDCD5 synergistically enhanced the apoptotic percentage of K562 cells induced by VP-16, as compared with that of Ad-null + VP16 and VP-16 alone respectively. It is concluded that Ad-PDCD5 may be a potential agent for enhancing the chemotherapy effect.
