Enhancement of Fas-mediated apoptosis in leukemic cell line HL-60 by Bay 11 - 7082.
- Author:
Li WANG
1
;
Ling-Bo LIU
;
Lei LI
;
Ping ZOU
Author Information
1. Department of Hematology, Union Hospital, Tongji Medical College, Huazhong University of science and technology, Wuhan 430022, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Down-Regulation;
Fas Ligand Protein;
physiology;
Gene Expression Regulation, Leukemic;
HL-60 Cells;
Humans;
NF-kappa B;
antagonists & inhibitors;
Nitriles;
pharmacology;
RNA, Messenger;
metabolism;
Sulfones;
pharmacology;
X-Linked Inhibitor of Apoptosis Protein;
metabolism;
fas Receptor;
metabolism
- From:
Journal of Experimental Hematology
2007;15(5):941-945
- CountryChina
- Language:Chinese
-
Abstract:
The aim of study was to explore the effects of NF-kappaB inhibitor Bay 11 - 7082 on Fas/FasL system and Fas-mediated apoptosis in HL-60 cells. The mRNA and protein expression levels of Fas, FasL and XIAP after treatment with Bay 11 - 7082 were detected by RT-PCR and FCM respectively. The level of sFasL was detected by ELISA before and after treatment with Bay 11 - 7082; apoptosis was detected by FCM before and after treatment with Bay 11 - 7082. The results showed that after treating HL-60 cells with Bay 11 - 7082, the mRNA and protein levels of FasL and XIAP were lower than that of controls, the difference was significant by statistic analysis (p < 0.05). Neither the mRNA and protein levels of Fas, nor the level of sFasL changed significantly (p > 0.05). Apoptotic rate of HL-60 cells treated with Bay 11 - 7082 was significantly higher as compared with controls (p < 0.05). It is concluded that Bay 11 - 7082 can enhance Fas-mediated apoptosis in HL-60 cells by down-regulation of FasL and XIAP levels.
