Influence of compound biejia ruangan prescription on extracelluar matrix in bleomycin induced pulmonary fibrosis rats.
- Author:
Dong-wei ZHANG
1
;
Ji-feng WANG
;
Jian-zhao NIU
;
Mei-juan YANG
;
Ling-qiao WANG
;
Huan JIN
;
Lian-qi LIU
;
Bao-hua GAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bleomycin; analogs & derivatives; Collagen Type III; blood; Collagen Type IV; blood; Drug Combinations; Drugs, Chinese Herbal; isolation & purification; pharmacology; Hyaluronic Acid; blood; Hydroxyproline; metabolism; Laminin; blood; Lung; metabolism; pathology; Male; Materia Medica; pharmacology; Plants, Medicinal; chemistry; Pulmonary Fibrosis; chemically induced; metabolism; pathology; Random Allocation; Rats; Rats, Sprague-Dawley
- From: China Journal of Chinese Materia Medica 2004;29(1):62-66
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the influence of compound Biejia Ruangan Prescription (CBRP) on extracelluar matrix in bleomycin induced pulmonary fibrosis rats.
METHOD54 male Sprague-Dawley rats were randomly divided into 6 groups (9 rats in each group). Rats in the model control group, positive medicine group, and high, moderate and low CBRP groups were injected with bleomycin A5 by trachea, and rats in sham-model control group with same volume normal saline. 29 days after the injection, CBRP solution of different dosages (1.4 g x kg(-1), 0.7 g x kg(-1), 0.35 g x kg(-1)) was respectively given to rats in the high, moderate and low CBRP group by gavage, while equal volume of normal saline was given to those in the sham-model control group and model control group, and an equal volume of prednisone (0.56 mg x kg(-1)) was given to those in positive medicine control group. On the 80th day, the levels of III-collagen, IV-collagen, laminin and hyaluronic acid in the serum were determined, the determination of hydroxyproline in lung homogenates was analyzed, and the right lung was incised to make pathological sections which were stained with Hematoxylin-Eosin (HE) and Masson staining for pathological diagnosis.
RESULTCBRP could decrease the levels of III-collagen, IV-collagen, laminin and hyaluronic acid in the serum.
CONCLUSIONCBRP may play its therapeutic role by leveling down the content of extracellular matrix in rats with pulmonary fibrosis induced by Bleomycin A5.
