The effect of Baicalin on gene expression profile in rat brain of focal cerebral ischemia.
- Author:
Zhong WANG
1
;
Kang YING
;
Zhan-jun ZHANG
;
Jian-xun LIU
;
Xiao-yan ZHANG
;
Li XU
;
Cui-e WEI
;
Yan HUANG
;
Yong-yan WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Brain Ischemia; genetics; metabolism; Flavonoids; isolation & purification; pharmacology; GTP-Binding Proteins; genetics; metabolism; Gene Expression Profiling; Gene Expression Regulation; drug effects; Male; Oligonucleotide Array Sequence Analysis; Plants, Medicinal; chemistry; Pyruvate Kinase; genetics; metabolism; RNA, Messenger; genetics; Rats; Rats, Sprague-Dawley; Scutellaria; chemistry; Vimentin; genetics; metabolism
- From: China Journal of Chinese Materia Medica 2004;29(1):83-86
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the difference of genes expression profiles between focal cerebral ischemia tissue and that treated with Baicalin using cDNA microarray.
METHODThe total RNAs were isolated from rat brains of sham-operation, vehicle (focal cerebral ischemia of rat brain) and baicalin-treated groups. mRNAs were reversely transcribed to cDNA with incorporation of fluorescent dUTP (Cy5 or Cy3 dUTP) to prepare hybridization probes. The PCR products of 4096 genes were spotted on the chip after a serial of treatment. The mixed probes were hybridized to the cDNA microarray. Axon Genepix 4000B and GenePixPro 3.0 software were used to scan and analyze the fluorescent signals.
RESULTThe expressions of 199 and 12 genes were found up-regulated and down-regulated, respectively, in the vehicle group compared with the sham-operation one. But the numbers of genes whose expressions were up-regulated and down-regulated were 89 and 88, respectively, when comparing the gene expression in the Baicalin-treated rat brain with that in the vehicle group. Moreover, one down-regulated and three up-regulated genes in the vehicle group were up-regulated and down-regulated in the Baicalin-treated group, respectively. Expressions of three up-regulated genes in the vehicle group were further reinforced in the Baicalin-treatment group.
CONCLUSIONMultiple pathways and nodes may be involved in the pharmacological effect of Baicalin on brain ischemia.
