Analysis of ant oil extracted with supercritical CO2 fluid from Polyrhachis vicina by GC-MS.
- Author:
Li-Rong SHEN
1
;
Yu-Cui REN
;
Feng-Qin FENG
;
Meng-Hao DU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Ants; chemistry; Carbon Dioxide; Chromatography, Supercritical Fluid; methods; Fatty Acids, Unsaturated; analysis; Gas Chromatography-Mass Spectrometry; Lead; analysis; Manganese; analysis; Materia Medica; chemistry; Oils, Volatile; chemistry; isolation & purification; Zinc; analysis
- From: China Journal of Chinese Materia Medica 2005;30(1):33-36
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the optimum extraction parameters and components on ant oil from Polyrhachis vicina.
METHODThe optimum condifious for supercritical CO2 fluid extraction (SFE-CO2), were investigated with orthogonal design, GC-MS was applied for analyzing. The components and their contents in the ant oil were analyzed by GC-MS, and the contents of lead, zinc and manganese in the oil were determined by ICP-AES.
RESULTThe optimum extraction parameters were achieved, temperature of 50 degrees C, pressure of 30 MPa and time of 2 hours. The extracting yield of the ant volatile oil was 11.4% - 14.3%. 51 Constituents were identified including 9-octadecenoic acid, ethyl oleate, cholesterol, n- Hexadecanoic acid, etc, and the content of various constituents was determined by orea normalization. The oil contained unsaturated fatty acid of 64.6%, lead of 0.80 microg x g(-1), zinc of 0.54 microg x g(-1) and manganese of 0.15 microg x g(-1).
CONCLUSIONThe method showes advantages including faster and efficient of extraction, good quality and no solvent residues in the oil.
