Determination of phillyrin and forsythoside A in Lianqiao Bingdu Qing capsule by RP-HPLC.
- Author:
Wen-Hua ZHAO
1
;
Ren-Bing SHI
;
Bin LIU
;
Lan-Zhen ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Capsules; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; administration & dosage; chemistry; isolation & purification; Forsythia; chemistry; Fruit; chemistry; Glucosides; analysis; Glycosides; analysis; Plants, Medicinal; chemistry
- From: China Journal of Chinese Materia Medica 2005;30(1):36-39
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish methods for the determination of phillyrin and forsythoside A in Lianqiao Bingdu Qing capsule by RP-HPLC.
METHODThe determination of phillyrin was carried out on YWG-C18 column (4.6 mm x 250 mm, 10 microm), using acetonitrile-water (25:75) as mobile phase at a flow rate of 0.8 mL x min(-1) and detected at the wavelength 277 nm. The determination of forsythoside A was carried out with YWG-C18 column(4.6 mm x 250 mm,10 microm), using acetonitrile-water-Acetic acid (17:83:0.4) as mobile phase at a flow rate of 1.0 mL x min(-1) and detected at the wavelength 280 nm.
RESULTThe average recovery of phillyrin was 99.6%, RSD = 1.9% (n = 5). The average recovery of forsythoside A was 101.3%, RSD = 2.5% ( n = 5).
CONCLUSIONThe methods were simple and accurate and could be used to control the quality of the Lianqiao Bingdu Qing capsule.
