Effects of allitridin on the expression of human cytomegalovirus immediate early antigens-IE72 and IE86 in human embryonic lung cells.
- Author:
Hong ZHEN
1
;
Feng FANG
;
Zhi-Feng LIU
;
Xing-Cao NIE
;
Wen CUI
;
Ge LI
Author Information
- Publication Type:Journal Article
- MeSH: Allyl Compounds; administration & dosage; isolation & purification; pharmacology; Antiviral Agents; pharmacology; Cells, Cultured; Cytomegalovirus; genetics; physiology; Fibroblasts; cytology; metabolism; Garlic; chemistry; Gene Expression Regulation, Viral; drug effects; Humans; Immediate-Early Proteins; metabolism; Injections; Lung; cytology; Sulfides; administration & dosage; isolation & purification; pharmacology; Trans-Activators; metabolism; Viral Proteins; metabolism
- From: China Journal of Chinese Materia Medica 2005;30(1):47-49
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of allitridin injection on the expression of human cytomegalovirus (HCMV) immediate-early antigens (IEAs including IE72 and IE86) in human embryonic lung cells.
METHODHCMV AD 169 Virus strain infected cell model (MOI = 2.5 and 0.25, respectively) were established, and then treated with ICm5 and MTC doses of allitridin. Western blot was used to analyze the of IE72 and IE86 expression after the treatment, ganciclovir(GCV, IC50 and 2.3 x IC50) treatment as control.
RESULTNo matter what kind of MOI was used, both IE86 and IE72 antigens' expression was effectively suppressed by allitridin treatment, and the inhibitory rate of IE86 was almost twice of IE72's. Compared with GCV, allitridin had stronger inhibitory effect on IE86 expressing, although its efficacy on IE72 was weaker than GCV.
CONCLUSIONAllitridin could suppress the expression of IE72 and IE86, especially for IE86 expressing, maybe it is ore of key role in the mechanism of allitridin against HCMV.
