Pseudolaric acid B induces human melanoma A375-S2 cell apoptosis in vitro.
- Author:
Xian-Feng GONG
1
;
Min-Wei WANG
;
Shin-ichi TASHIRO
;
Satoshi ONODERA
;
Takashil IKEJIMA
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Apoptosis Regulatory Proteins; Cell Line, Tumor; Diterpenes; isolation & purification; pharmacology; Dose-Response Relationship, Drug; Humans; Melanoma; metabolism; pathology; Pinaceae; chemistry; Proteins; metabolism; Proto-Oncogene Proteins c-bcl-2; metabolism; bcl-2-Associated X Protein; bcl-X Protein
- From: China Journal of Chinese Materia Medica 2005;30(1):55-57
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the mechanisms of pseudolaric acid B-induced apoptosis on A375-S2 cells.
METHODMTT, fluorescence microscope observation, DNA agarose gel electrophoresis and Western blot analysis wereused.
RESULTPseudolaric acid Binduces A375-S2 cell apoptosis in a time and dose-dependent manner. Apoptotic bodies and DNA ladder were observed in 5 micromol x L(-1) pseudolaric acid B-treated A375-S2 cells for 36 h. The expression of Bcl-2, Bcl-xL and ICAD was reduced time dependently, whereas the expression of Bax was increased.
CONCLUSIONThe major cause of pseudolaric acid B induced cytotoxicity on A375-S2 cells was apoptosis. Mitochondria proteins and ICAD might be involved in the apoptotic pathways of pseudolaric acid B-treated A375-S2 cells.
