Studies on TLC fingerprint of flavonoids in rhizome of Polygonatum odoratum.
- Author:
Xian-guo YANG
1
;
Si-bao CHEN
;
Shi-lin CHEN
;
Da-jian YANG
;
Ta-si LIU
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, Thin Layer; methods; Flavonoids; analysis; chemistry; Plants, Medicinal; chemistry; Polygonatum; chemistry; Quality Control; Rhizome; chemistry
- From: China Journal of Chinese Materia Medica 2005;30(2):104-106
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the thin layer chromatographic (TLC) fingerprint of flavonoid constituents from Polygonatum odoratum, to set up the identification protocol of the herbal and provide scientific information for its quality control.
METHODThe ethanol extracts were separated on silica gel G precoated plate with a mixture of toluene-ethylacetate-formic acid (5:4:1) as the mobile phase. The spots were visualized with ammonia vapor, then were examined under ultraviolet light (365 nm). The plate was scanned at wavelengths of lambdaR = 500 nm, lambdaS = 280 nm.
RESULTA fingerprint of flavonoids of P. odoratum, with 10 specific fluorescent spots while examined under ultraviolet light, was set up.
CONCLUSIONThe method can be used for quality control of P. odoratum.
