Determination of gallic acid in Melastoma dodecandrum by RP-HPLC.
- Author:
Xun HE
1
;
Yong-jun LI
;
Li-na LIU
;
Yan-yu LAN
;
Ai-min WANG
;
Yong-lin WANG
Author Information
- Publication Type:Journal Article
- MeSH: China; Chromatography, High Pressure Liquid; methods; Ecosystem; Gallic Acid; analysis; Melastomataceae; chemistry; Plants, Medicinal; chemistry; Quality Control
- From: China Journal of Chinese Materia Medica 2005;30(3):180-181
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a RP-HPLC method for the determination of gallic acid in Chinese herb Melastoma dodecandrum, and to evaluate the quality of the herb.
METHODThe RP-HPLC analysis was achieved by using a Polaris C18 column and tetrahydrofuran-methanol-0.2% phosphoric acid (0.5:0.5:99) as the mobile phase, with a flow rate of 1.0 mL x min(-1), and detected by UV at 274 nm.
RESULTThe content of gallic acid was from 0.020% to 0.081%, in ten groups of M. dodecandrum collected from different locations.
CONCLUSIONThe method is a simple, convenient and rapid. It can be used for quality evaluation of M. dodecandrum.
