HPLC analysis of alfatoxins in medicinal herb extracts by immunoaffinity column cleanup and post-column bromination.
- Author:
Xue-hui ZHANG
1
;
Jian-min CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Aflatoxin B1; analysis; Aflatoxins; analysis; Chromatography, Affinity; Chromatography, High Pressure Liquid; methods; Codonopsis; chemistry; Drugs, Chinese Herbal; chemistry; Plants, Medicinal; chemistry; Prunus; chemistry
- From: China Journal of Chinese Materia Medica 2005;30(3):182-184
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a new and accurate method to quantify aflatoxins in medicinal herbs.
METHODThis method consists of sample extraction by using MeOH-H2O (7:3), followed by clean-up with an immunoaffinity column, and finally HPLC determination with fluorescence detection. Aflatoxins B1 and G1 are determined as their bromine derivatives, produced in an on-line post-column derivatization system.
RESULTThe overall average recoveries for different medicinal herbs spiked at two levels of standards were from 90.4% to 99.7%. The detection limit was 0.06 microg x kg(-1) for aflatoxins G2 and B2, and 0.20 microg x kg(-1) for aflatoxins G1 and B1.
CONCLUSIONThe use of immunoaffinity column provides excellent cleanup of interfering substances. The method has been applied successfully to analyze 96 natural drugs.
