Determination of linarin in Yuye Jiedu granule by RP-HPLC.
- Author:
Dan LU
1
;
Gao-rong LU
;
Jie-ming ZOU
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Chrysanthemum; chemistry; Drug Combinations; Drugs, Chinese Herbal; administration & dosage; chemistry; isolation & purification; Glycosides; analysis; Lonicera; chemistry; Plants, Medicinal; chemistry; Quality Control
- From: China Journal of Chinese Materia Medica 2005;30(3):191-192
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the content of linarin in Yuye Jiedu granule.
METHODA HPLC method was developed. The chromatographic conditions are as follows Luna C18 column and acetonitrile-0.05 mol x L(-1) phosphate buffer-phosphoric acid (30:70:0.06) as mobil phase, detection wavelenth at 327 nm.
RESULTThe linear range of linarin was 0.025-0.50 microg. The average recovery was 98.7% and RSD 2.7%.
CONCLUSIONThe method is simple and accurate, with good repeatability, and can be used for determination of linarin in Yuye Jiedu granule.
