Alteration of activities of telomerase in tanshinone IIA inducing apoptosis of the leukemia cells.
- Author:
Yi SONG
1
;
Shu-lan YUAN
;
Yi-ming YANG
;
Xiu-jie WANG
;
Guang-qi HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Cell Proliferation; drug effects; Diterpenes, Abietane; HL-60 Cells; Humans; K562 Cells; Phenanthrenes; isolation & purification; pharmacology; Plants, Medicinal; chemistry; Proto-Oncogene Proteins c-bcl-2; metabolism; Proto-Oncogene Proteins c-fos; metabolism; Proto-Oncogene Proteins c-myc; metabolism; Salvia miltiorrhiza; chemistry; Telomerase; metabolism; Tumor Suppressor Protein p53; metabolism
- From: China Journal of Chinese Materia Medica 2005;30(3):207-211
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of tanshinone IIA on HL-60 and K562 cells apoptosis, and to assay the inhibition of the telomerase activities in the leukemia cell apoptosis induced by Tanshinone.
METHODUsing the techniques of cell culture in vitro, flow cytometry and PCR-TRAP observed the telomerase activities and apoptosis of HL-60 and K562 cells which treated by Tan IIA.
RESULT0.5 microg x mL(-1) Tan IIA could obviously inhibit HL-60 and K562 cell lines growth (P < 0.05), down-regulate c-myc, bcl-2 gene and up-regulate c-fos and p53 gene expression as well as induce leukemia cell apoptosis, the apoptotic rates of HL-60 and K562 cells were 11.8% and 21.8% respectively. The telomerase activities significant decreased, the inhibiting rates in HL60 and K562 cells were 30.8% and 50.8% respectively.
CONCLUSIONTan IIA could significantly inhibit the proliferation and telomerase activities of HL-60 and K562 cells and induce the leukemia cell apoptosis.
