Determination of ligustilide in volatile oil from rhizome of ligusticum chuanxiong by RP-HPLC.
- Author:
Yan-zhi LIN
1
;
Xing TANG
;
Kai-shun BI
Author Information
- Publication Type:Journal Article
- MeSH: 4-Butyrolactone; analogs & derivatives; analysis; Chromatography, High Pressure Liquid; methods; Ligusticum; chemistry; Oils, Volatile; chemistry; isolation & purification; Plants, Medicinal; chemistry; Rhizome; chemistry
- From: China Journal of Chinese Materia Medica 2004;29(2):154-157
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine Ligustilide in volatile oil from Ligustrcum chuanxiong with RP-HPLC.
METHODODS2 column (4.6 mm x 200 mm, 5 microm) was used and nitrendipine was used as internal standard. The mobil phase consisted methanol, acetontrile and water (33:21:46). The ligustilide was at 275 nm.
RESULTThe linear range was 2.92-29.2 mg x L(-1) for ligustilide. The average recovery of ligustilide was 95.1% and RSD was 2.3%.
CONCLUSIONThis method is simple and can be used to determine ligustilide with satisfactory accuracy and reproducibility.
