Influence of mutation in HBV precore region on the expression of HLA-I in HepG2 cells.
- Author:
Weihong CHEN
1
;
Yulong LIN
;
Kangxian LUO
;
Jinlin HOU
Author Information
- Publication Type:Journal Article
- MeSH: DNA, Viral; HLA Antigens; immunology; Hep G2 Cells; Hepatitis B e Antigens; Hepatitis B virus; genetics; Humans; Mutation; Polymerase Chain Reaction
- From: Chinese Journal of Hepatology 2002;10(5):351-353
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the influence of host cellular HLA-I molecules expression by HBV precore region mutants.
METHODSThe plasmids of the wild type of HBV precore region and the mutants of A83 and A83/A86 were constructed, and then transected into HepG2 cells. The biological activity of HBV precore gene in the host cells was identified. The expression of HLA-I molecules was detected by flow cytometric analysis.
RESULTSDNA segments similar with HBV precore gene size and HBeAg were detected by PCR and ELISA, respectively. The fluorescence intensity of HLA-I on host cells was different: wild type being 1.3; A83, 17.6; and A83/A86, 7.3.
CONCLUSIONSHBV precore gene hot-spot mutants in vitro can increase the expression of HLA-I molecules on host cells.
