Synergetic transactivating functions of HCV core and truncated HBV middle surface protein.
- Author:
Yan LIU
1
;
Jun CHENG
;
Gang WANG
;
Ke LI
;
Huijuan DUAN
;
Lin WANG
;
Li LI
;
Ling Xia ZHANG
;
Jumei CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Hep G2 Cells; Hepatitis B; genetics; metabolism; Hepatitis C; genetics; metabolism; Humans; Membrane Proteins; genetics; metabolism; Plasmids; Promoter Regions, Genetic; Transcriptional Activation; Transfection; Viral Core Proteins; genetics; metabolism; beta-Galactosidase
- From: Chinese Journal of Hepatology 2002;10(5):354-357
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the synergetic transactivating functions of HCV core and truncated HBV middle surface proteins.
METHODSTwo recombinant expression plasmids harboring HCV core and C-terminally truncated HBV middle surface protein gene were constructed, respectively. The plasmids were transfected into HepG2 cells and cotransfected HepG2 cells with reporter plasmid pSV-lacZ by lipofectamine plus reagents. The transient expressed viral proteins were identified at the transcription and translation levels. The activity of beta-galactosidase was detected, which reflected the transactivating function of the proteins.
RESULTSThe protein expression of plasmids was detected in soluble cell extracts of transiently transfected HepG2 cells. HCV core protein activated the beta-galactosidase expression at a value of 4.6 times higher than the control, while C-terminally truncated HBV middle surface protein activated at a value of 3.2 times. It reached 8.4 times transfected with the plasmids simultaneously. The transactivating effect was dose dependent.
CONCLUSIONSIt is suggested that the two kinds of virus proteins have transactivating effect on SV40 early promoter/enhancer, and they act synergistically. These contribute to explain the mechanisms of liver injury or tumorigenesis induced by HCV or/and HBV infection.