Study on the effects of combined IL-12 and GM-CSF gene therapy for murine liver cancer.
- Author:
Shuangjian QIU
1
;
Shenglong YE
;
Zhong WANG
;
Zhaoyiu TANG
;
Lina LU
;
Xiao XIAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; CD4-Positive T-Lymphocytes; cytology; CD8-Positive T-Lymphocytes; cytology; Cell Division; genetics; physiology; Genetic Therapy; methods; Granulocyte-Macrophage Colony-Stimulating Factor; blood; genetics; physiology; Interferon-gamma; blood; Interleukin-2; blood; genetics; physiology; Liver Neoplasms, Experimental; genetics; therapy; Lymphocyte Count; Male; Mice; Mice, Inbred BALB C; Neoplasm Transplantation; Plasmids; administration & dosage; genetics; Time Factors; Tumor Cells, Cultured
- From: Chinese Journal of Hepatology 2002;10(6):413-416
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the anti-tumor effects of combined IL-12 and granalocyte-macrophage-colong scimulating factor (GM-CSF) gene therapy on murine hepatocellular carcinoma.
METHODSTwenty-four mice received subcutaneous inoculation of 1 x 10(6) BNL hepatoma cells were randomly divided into the following four groups with different cytokine encoding plasmids (6 mice for each group): (1)pXX-GM-CSF 12.5 microg and pXX-IL-12 12.5 microg; (2)pXX-IL-12 25 microg; (3)pXX-GM-CSF 25 microg; (4)pXX-Neo 25 microg. The plasmids were given through tail vein using a versatile hydrodynamics-based DNA delivery method on day 3 and day 6 after tumor challenge. The growth of tumor and cellular immune response were observed intensively. The changes in serum concentration of IL-12, GM-CSF, and IFN-gamma after plasmids injection were also observed.
RESULTSCo-delivery of IL-12 and GM-CSF could mount stronger anti-tumor effects, longer term enhanced IL-12 expression and lower level of IFN-gamma than did IL-12 alone.
CONCLUSIONSCombined IL-12 and GM-CSF can render a strong anti-tumor effect as well as a potential to lower the side effects.
