OBJECTIVETo investigate the measurements of gene expressing at a single hepatocyte level.

METHODSIndividual hepatocyte was isolated from cryostat tissue section using laser microdissection technique. To detect the mRNA expressed by single hepatocyte, RNA was extracted, reversely transcribed to cDNA and amplified by nested polymerase chain reaction (PCR).

RESULTSSingle cell was microdissected from cryostat tissue using an ultraviolet laser micromanipulator. The RNA could be extracted from the isolated cell(s), and the RT-PCR production could be observed after electrophoresis, whose quantitation was compatible with the number of cells.

CONCLUSIONCombining laser microdissection and nested RT-PCR can monitor gene expression at a single cell level in vivo.