Mitogenic effects of growth and differentiation factors on rat liver stem cell WB-F344 in vitro.
- Author:
Peng YAO
1
;
Yi-qiong ZHAN
;
Wang-xiang XU
;
Chang-yan LI
;
Xiao-ming YANG
;
Da-rong HU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Differentiation; drug effects; Cell Division; drug effects; Cytokines; pharmacology; Epidermal Growth Factor; pharmacology; Hepatocyte Growth Factor; pharmacology; Insulin; pharmacology; Liver; cytology; Rats; Rats, Inbred F344; Stem Cells; cytology; drug effects; Transforming Growth Factor beta; pharmacology
- From: Chinese Journal of Hepatology 2003;11(1):33-36
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the relationship between the proliferation, differentiation of rat hepatic stem like cell line WB-F344 and cytokines in vitro.
METHODS(3)H thymidine labelling of new synthesized DNA was used to examine the mitogenic responsiveness of WB-F344 cells to cytokines, western blot was used to study the expression of cytokines receptors on hepatic stem cells, and apoptotic cells were detected by Flow cytometry.
RESULTSWB-F344 cells showed a proliferative response to the cytokines of hepatocyte growth factor (HGF), epidermal growth factor (EGF), fibroblast growth factor (FGF), Insulin at the dose of 80 ng/ml, and the relative cpm values are 982.95, 906.32, 863.98 and 968.67 respectively, while non response to interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha) at the same dose, and an inhibition or apoptosis response to transforming growth factor-beta (TGF-beta) at 80 ng/ml with a 26.89% apoptotic rate. Western blot showed that there were HGF, EGF, FGF, TGF-beta receptors expressed on WB-F344 cells. When WB-F344 cells were cultured in the differential system (DMEM, 10% Fcs, HGF 10 to approximately 50 ng/ml, EGF 20 ng/ml, Insulin 1 microg/ml, Dex 1 micromol/L), the cells could differentiated into hepatocytes. In addition, HGF could scattered WB-F344 cells.
CONCLUSIONThe proliferation and differentiation of liver stem cells are regulated by various cytokines which may play an important role when liver is damaged seriously.