Regulation of NF-κB signal transduction pathway on cytokines in cultured nasal epithelium
10.3760/cma.j.issn.1673-0860.2010.07.017
- VernacularTitle:核因子κB信号转导通路对鼻黏膜上皮细胞的细胞因子调控
- Author:
Ji-Hong YANG
1
;
Yuan LI
;
Ge-Hua ZHANG
;
Qin-Tai YANG
Author Information
1. 中山大学附属第三医院
- Keywords:
Nasal mucosa;
Epithelial cells;
NF-kappa B;
Interleukins;
Adaptor proteins,signal transducing
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2010;45(7):592-596
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the activation and regulation of nuclear factor-kappa B (NF-κB)on transcription of cytokines in cultured lipopolysaccharide (LPS) -induced nasal epithelial cells. Methods Normal sphenoid mucosa epithelium from 11 patients who accepted pitaitary tumor surgery via trans-sphenoid approach was separated and cultured without serum. The epithelium of the third or the forth passage was induced with LPS. Wedelolactone, a blocking agent of NF-κB was used at the same time. An electrophoretic mobility shift assay was used to detect DNA-binding activity of NF-κB. The reverse transcription-polymerase chain reaction was used to evaluate mRNA of interleukin-1β( IL-1β), tumor necrosis factor-α(TNF-α),IL-5, IL-6, IL-8, granulocyte-macrophage colony stimulating factor ( GM-CSF), regulated on activation,normal T expressed and secreted (RANTES), eotaxin, eotaxin-2, vascular cell adhesion molecule-1 ( VCAM-1 ), intercellular adhesion molecule-1 ( ICAM-1 ), inducible nitric oxide synthase ( iNOS ) and cyclooxygenase-2(COX-2). Statistical analysis was performed using SPSS17.0 software. Results DNA binding activity of NF-κB and mRNA of IL-1β, IL-8 and COX-2 increased in cultured LPS-induced nasal epithelial cells (relative values were 1. 013 ±0. 144, 0, 0, 0 respectively in control group and relative ones of LPS-induced were 2. 050 ±0. 305, 1. 057 ±0. 041,0. 950 ±0. 042, 0. 117 ±0. 012 respectively). There was significant difference between the control group and LPS-induced nasal epitherial cells group( P values were 0. 004, 0. 000, 0. 000, 0. 000 respectively). Corresponding expression of NF-κB, IL-1β, IL-8 and COX-2 decreased after the addition of Wedelolactone(relative values were 0. 917 ±0. 188, 0. 180 ±0. 008,0, 0 respectively). There was significant difference between the LPS-induced nasal epithelial cells group and the Wedelolactone-addition group ( P values were 0. 002, 0. 000, 0. 000, 0. 000 respectively ). But the expression of mRNA of other factors were 0 in all groups. Conclusions The NF-κB signal transduction pathway was involved in the transcriptional regulation of IL-1β, IL-8 and COX-2 in cultured LPS-induced nasal epithelial cells.
