Association of mutations of HFE gene and hepatocellular carcinoma following chronic hepatitis B.
- Author:
Wen-juan SHI
1
;
Hong CHEN
;
Bin ZHOU
;
Jun CHENG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Carcinoma, Hepatocellular; complications; genetics; Female; Hemochromatosis Protein; Hepatitis B, Chronic; complications; genetics; Histocompatibility Antigens Class I; genetics; Humans; Liver Neoplasms; complications; genetics; Male; Membrane Proteins; genetics; Middle Aged; Mutation
- From: Chinese Journal of Hepatology 2005;13(9):682-684
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the frequency of HFE gene variants in patients with hepatocellular carcinoma following chronic hepatitis B and to analyze their relationships.
METHODS56 patients with hepatocellular carcinoma following chronic hepatitis B (HCC group) and 60 healthy blood donors (control group) were studied for the amino acid dimorphism at codon 63 (His63Asp=H63D) and codon 282 (Cys282Tyr = C282Y) of the HFE gene. The codon 63 and 282 dimorphisms were defined by PCR amplification of genomic DNA samples and restriction enzyme digestion using RsaI for C282Y and BclI for H63D. The association between hepatocellular carcinoma following chronic hepatitis B and HFE mutations were analyzed by Chi-square test.
RESULTSThe genotype frequency of C2/C2 in the HCC group was markedly higher than that in the normal control group (10.7% vs 0) and there was a significant correlation between them. At the same time, the allele frequency of C2 in the HCC group was markedly higher than that in the normal control group (16.1% vs 1.7%) and there was a significant correlation between them also.
CONCLUSIONThe mutation of C282Y may be related with susceptibility to HCC after chronic hepatitis B. This outcome suggests that host HFE mutation may be an important factor related to the pathogenesis of HCC.
