The expression and the significance of L-FABP and FATP4 in the development of nonalcoholic fatty liver disease in rats.
- Author:
Ai-juan FENG
1
;
Dong-feng CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Fatty Acid Transport Proteins; biosynthesis; genetics; Fatty Acid-Binding Proteins; biosynthesis; genetics; Fatty Liver; metabolism; Male; RNA, Messenger; biosynthesis; genetics; Random Allocation; Rats; Rats, Wistar
- From: Chinese Journal of Hepatology 2005;13(10):776-779
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of liver fatty acid binding protein(L-FABP) and fatty acid transport protein (FATP4) in the development of nonalcoholic fatty liver disease (NAFLD) in rats.
METHODSThe expression of L-FABP and FATP4 genes was examined in fatty liver rats by reverse transcription and polymerase chain reaction amplification and Western blot methods.
RESULTSIn the high fat diet group (F), mRNA and protein expression of L-FABP and FATP4 were increased at 2 weeks, and they increased remarkably at 12 weeks (P < 0.05; L-FABP mRNA F=124.9, protein expression F=92.6; FATP4 mRNA F=602.9, protein expression F=108.8).
CONCLUSIONThe high expression of L-FABP and FATP4 at the early stage is an adaptive reaction of the body, With the advanced expression of the L-FABP and FATP4, it can lead to a fatty acid disequilibrium and then result in nonalcoholic fatty liver disease in the rats.
