A method of HPRE synthesis via transcription by T7 RNA polymerase in vitro.
- Author:
Ying HUANG
1
;
Jin-jun GUO
;
Jun ZHANG
;
Wei-xian CHEN
;
Ai-long HUANG
Author Information
- Publication Type:Journal Article
- MeSH: DNA-Directed DNA Polymerase; DNA-Directed RNA Polymerases; Hepatitis B virus; genetics; RNA Processing, Post-Transcriptional; RNA Splicing; RNA-Binding Proteins; physiology; Transcription, Genetic; Viral Proteins
- From: Chinese Journal of Hepatology 2005;13(11):808-810
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo synthesize highly pure HBV post-transcriptional regulatory element (HPRE) via transcription in vitro by T7 RNA polymerase.
METHODSHPRE gene was amplified by PCR from a template containing HBV complete genomic DNA and cloned into plasmid pGEM-11zf. The cloned DNA sequence was transcribed by T7 RNA polymerase.
RESULTSThe construction of HPRE gene recombinant plasmid and production of HPRE via transcription in vitro was successful.
CONCLUSIONIn vitro transcription by T7 RNA polymerase can be used to synthesize highly pure HPRE.
