Effects of glycyrrhizin on TGFbeta1 stimulated hepatic stellate cell signaling transduction.
- Author:
Ling DONG
1
;
Jian-yong SUN
;
Guo-ting FANG
;
Lin-di JIANG
;
Ji-yao WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Glycyrrhizic Acid; pharmacology; Hepatocytes; metabolism; Male; Rats; Signal Transduction; Smad Proteins; metabolism; Transforming Growth Factor beta; pharmacology
- From: Chinese Journal of Hepatology 2005;13(11):828-831
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo investigate the role of glycyrrhizin on TGFbeta1 stimulated signaling transduction in rat hepatic stellate cells (HSCs).
METHODSThe mice HSCs were isolated and cultured with or without glycyrrhizin (1 micromol/L-1000 micromol/L) in vitro after TGFbeta1 stimulation. The mRNA level of Smad2, 3, 7 were measured with RT-PCR; protein expression level of Smad2, 3, 7 and collagen I, III were analyzed with Western blot.
RESULTSTGFbeta1 increased the mRNA level and protein expression of Smad2, 3, 7 in HSC; it also increased protein expression of collagen I and III. 1 micromol/L-1000 micromol/L glycyrrhizin decreased the mRNA level and protein expression of Smad2, 3, 7; it also inhibited protein expression of collagen I and III gradually.
CONCLUSIONInterventing the TGFbeta signaling pathway and decreasing the synthesis of collagen, might be involved in the anti-fibrosis mechanism of glycyrrhizin.
