Neuroprotective effect of longistyline A against corticosterone-induced neurotoxicity in PC12 cells.
- Author:
Bao-Ping JIANG
1
;
Rui-Wu YANG
;
Xin-Min LIU
;
Ya-Min LIU
;
Qi CHANG
;
Jian-Yong SI
;
Rui-Le PAN
Author Information
1. Sichuan Agricultural University, Ya 'an 625014, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Cajanus;
chemistry;
Calcium;
metabolism;
Caspase 3;
metabolism;
Cell Survival;
drug effects;
Corticosterone;
toxicity;
DNA Fragmentation;
drug effects;
L-Lactate Dehydrogenase;
metabolism;
Molecular Structure;
Neuroprotective Agents;
isolation & purification;
pharmacology;
PC12 Cells;
Phenols;
isolation & purification;
pharmacology;
Plant Leaves;
chemistry;
Plants, Medicinal;
chemistry;
Rats
- From:
Acta Pharmaceutica Sinica
2012;47(5):600-603
- CountryChina
- Language:Chinese
-
Abstract:
This study is to investigate the protective effect of longistyline A against corticosterone-induced neurotoxicity in PC12 cells. While PC12 cells were exposed to 100 micromol x L(-1) corticosterone for 48 h, cell survival rate was reduced and lactate dehydrogenase (LDH) release increased. In parallel, corticosterone caused significant elevations of DNA fragmentation, [Ca2+]i and caspase-3 activity. However, when the PC12 cells were incubated with longistyline A (4.0, 8.0 and 16.0 micromol x L(-1)) in the presence of 100 micromol x L(-1) corticosterone for 48 h, the effects were evidently alleviated, but dose-dependent manner was not obvious. In summary, longistyline A could generate a neuroprotective effect against corticosterone-induced neurotoxicity in PC12 cells possibly by decreasing [Ca2+]i and caspase-3 activity.
