Triterpenoids from Inonotus obliquus protect mice against oxidative damage induced by CCl4.
- Author:
Fen-Qin ZHAO
1
;
Lin YAN
;
Xian-Hong CUI
;
Sheng LIN
;
Cong WANG
;
Hui ZHANG
;
Xiao-Yan KANG
;
Bian-Sheng JI
Author Information
1. Pharmaceutical Institute of Henan University, Kaifeng 475004, China.
- Publication Type:Journal Article
- MeSH:
Alanine Transaminase;
blood;
Animals;
Aspartate Aminotransferases;
blood;
Carbon Tetrachloride;
Catalase;
blood;
metabolism;
Female;
Glutathione;
blood;
metabolism;
Glutathione Peroxidase;
blood;
metabolism;
Interleukin-6;
blood;
Lanosterol;
analogs & derivatives;
isolation & purification;
pharmacology;
Liver;
metabolism;
Male;
Malondialdehyde;
blood;
metabolism;
Mice;
Oxidative Stress;
drug effects;
Polyporaceae;
chemistry;
Protective Agents;
isolation & purification;
pharmacology;
Random Allocation;
Superoxide Dismutase;
blood;
metabolism;
Triterpenes;
isolation & purification;
pharmacology
- From:
Acta Pharmaceutica Sinica
2012;47(5):680-684
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effects of lanosterol (1), inotodiol (2) and trametenolic acid (3) from Inonotus obliquus against oxidative damage induced by CCl4 in mice, 1, 2 and 3 (20, 10 and 5 mg x kg(-1)) were respectively administered to mice, once a day for 3 days. Then the mice were induced to oxidative damage by CCl4 on the third day 30 min after the administration. The activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-PX) and the content of malondialdehyde (MDA) and reductive glutathione (GSH) in serum and liver homogenate were determined. And the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and interleukin-6 (IL-6) concentration in serum were detected. The results showed that treatment with compound 1, 2 and 3 could significantly increase the activities of SOD, CAT and GSH-PX in serum and liver homogenate. Furthermore, the content of GSH in serum and liver homogenate increased and MDA content decreased markedly. In addition, compound 1, 2 and 3 could significantly inhibit the activities of ALT and AST in serum, and decrease the IL-6 concentration in serum remarkably. So, compound 1, 2 and 3 can protect mice against oxidative stress injury induced by CCl4. Furthermore, compound 1, 2 and 3 can protect cells from damage through inhibition on ALT, AST and the expression of IL-6.
