Differential Expression of TGF-beta Isoforms During Differentiation of HaCaT Human Keratinocyte Cells: Implication for the Separate Role in Epidermal Differentiation.
10.3346/jkms.2004.19.6.853
- Author:
Hang Rae CHO
1
;
Seok Beom HONG
;
Young Il KIM
;
Jin Woo LEE
;
Nack In KIM
Author Information
1. Department of Dermatology, College of Medicine, Kyung Hee University, Seoul, Korea. nikim@khmc.or.kr
- Publication Type:Original Article
- Keywords:
Transforming Growth Factor beta;
Cell Differentiation;
Keratinocytes;
Calcium
- MeSH:
Cell Differentiation/physiology;
Cell Line;
Gene Expression Regulation, Developmental/*physiology;
Humans;
Keratinocytes/*cytology/*physiology;
Protein Isoforms/metabolism;
Transforming Growth Factor beta/*metabolism
- From:Journal of Korean Medical Science
2004;19(6):853-858
- CountryRepublic of Korea
- Language:English
-
Abstract:
The three mammalian isoforms of transforming growth factor-beta (TGF-beta1, beta2, beta3) are potent regulators of cell growth, differentiation, and extracellular matrix deposition. To study their role in skin differentiation, we investigated the expression of TGF-beta isoforms on cell growth and differentiation induction of the human keratinocyte cell line, HaCaT by elevating the Ca2+ concentration. An ELISA and RT-PCR assay revealed secreted TGF-beta 1 protein and TGF-beta1 mRNA were increased during calci-um-induced differentiation. In contrast, major differences were seen for TGF-beta 2 and TGF-beta 3 mRNA which were decreased during differentiation, but TGF-beta 2 and TGF-3beta protein were not evident on an ELISA. These results suggest different functions for each TGF-beta isoforms in epidermal differentiation, such that TGF-beta 1 is associ-ated with the more differentiated state, and TGF-beta 2 and TGF-beta 3 may be associ-ated the more proliferated state.
