Effects of jin chai antiviral capsule on IFITM3 expression in mice.
- Author:
Ju-Ying ZHONG
1
;
Xiao-Lan CUI
;
Yu-Jing SHI
;
Shan-Shan GUO
;
Ying LIU
;
Fang-Zhou LIU
;
Ying-Jie GAO
;
Ya-Hong JIN
;
Hong-Xin CAO
Author Information
1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Antiviral Agents;
administration & dosage;
pharmacology;
Capsules;
Dose-Response Relationship, Drug;
Drug Combinations;
Drugs, Chinese Herbal;
administration & dosage;
pharmacology;
Female;
Influenza A Virus, H1N1 Subtype;
drug effects;
Lung;
metabolism;
Male;
Membrane Proteins;
metabolism;
Mice;
Mice, Inbred ICR;
Orthomyxoviridae Infections;
metabolism;
virology;
Plants, Medicinal;
chemistry;
Pneumonia;
metabolism;
virology;
Viral Load;
drug effects
- From:
Acta Pharmaceutica Sinica
2012;47(7):904-908
- CountryChina
- Language:Chinese
-
Abstract:
This study is to investigate the treatment of Jin Chai antiviral capsule for influenza virus FM1/47 (H1N1) infection. The model of pneumonia was established by dropping influenza virus into the nose of normal mice, real-time PCR and Western blot technique were used to detect the virus load and the interferoninducible transmembrane protein3 (IFITM3) in lung of mice at the 1st day, 3rd day, 5th day and 7th day after affected. The results showed that Jin Chai antiviral capsule in large, middle, small dose groups can decrease virus load significantly at each time point, after being affected (P<0.05, P<0.01), Jin Chai antiviral capsule can increase the interferoninducible transmembrane protein3 in lung of mice, large dose groups are significantly higher in expression of IFITM3 compared with model group at each time point (P<0.05, P<0.01). Middle dose groups are significantly higher in expression of IFITM3 compared with model group at the 3th day and the 5th day (P<0.05), small dose groups are significantly higher in expression of IFITM3 compared with model group at the 3th day (P<0.05). It can be concluded that Jin Chai antiviral capsule exerts antiviral effects against influenzavirus by raised expression of IFITM3.
