Analysis of primary metabolites of ranolazine in dog urine by LC-MS(n).
- Author:
Jing-ya TANG
1
;
Wei WANG
;
Li LI
;
Chun-gang ZHANG
;
Di-min WANG
;
Lu SUN
Author Information
1. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
- Publication Type:Journal Article
- MeSH:
Acetanilides;
administration & dosage;
metabolism;
urine;
Administration, Oral;
Animals;
Chromatography, Liquid;
Dogs;
Female;
Male;
Piperazines;
administration & dosage;
metabolism;
urine;
Ranolazine;
Solid Phase Extraction;
Spectrometry, Mass, Electrospray Ionization;
Tandem Mass Spectrometry
- From:
Acta Pharmaceutica Sinica
2012;47(8):1031-1038
- CountryChina
- Language:Chinese
-
Abstract:
Ranolazine and metabolites in dog urine were identified by LC-MS(n). Dog urine samples were collected after ig 30 mg x kg(-1) ranolazine, then the samples were enriched and purified through solid-phase extraction cartridge. The purified samples were analyzed by LC-MS(n). The possible metabolites were discovered by comparing the full scan and SIM chromatograms of the test samples with the corresponding blanks. Seventeen phase I metabolites and fourteen phase II metabolites were identified in dog urine. Three metabolites were identified by comparing with the control article. The metabolites were formed via the following metabolic pathways: O-demethylation, O-dearylation, hydroxylation, N-dealkylation, amide hydrolysis, glucuronidation and sulfation. The LC-MS(n) method is suitable for the rapid identification of drug and its metabolites in biologic samples.
