Study of target pegylated recombinant mutant human granulocyte colony stimulating factor.
- Author:
Yan-Shan HUANG
1
;
Rong JIN
;
Xiao-Ni LIU
;
Chang-Mei WANG
;
Ya-Wen RONG
;
Zhi CHEN
Author Information
1. Institute of Infectious Diseases, First Affiliated Hospital of Medical College, Zhejiang University, Hang Zhou 310003, China.
- Publication Type:Journal Article
- MeSH:
Amino Acid Sequence;
Base Sequence;
Chromatography, Ion Exchange;
Granulocyte Colony-Stimulating Factor;
biosynthesis;
chemistry;
genetics;
Humans;
Molecular Sequence Data;
Mutant Proteins;
biosynthesis;
genetics;
Polyethylene Glycols;
chemistry;
Protein Sorting Signals;
Recombinant Proteins
- From:
Chinese Journal of Biotechnology
2007;23(5):919-923
- CountryChina
- Language:Chinese
-
Abstract:
Recombinant mutant human granulocyte colony stimulating factor (rmhG-CSF) was pegylated, purified and characterized. rhG-CSF was mutated in position 1,3,4,5,17, and cysteine was added in C-terminal. rmhG-CSF was pegylated by PEG-Mal 20000 and separated by ion-exchange chromatography, gel filtration chromatography. Analysis of SDS-PAGE showed thar the purity of the separated PEG-rmhG-CSF was greater than 95%. and in intro and in vivo bioactivity study showed that target modified PEG-rmhG-CSF kept full bioactivity which was better than traditional pegylation method, and longer half-life was proved in mice.
