Effects of short-term continuous shear stress on cells growth and recovery of Laminaria japonica gametophytic cells in photobioreactor.
- Author:
Na HUANG
1
;
Si-Ye CHEN
;
Han-Shi QI
Author Information
1. Center of Cell Culture and Bioprocess Engineering, Shanghai Jiaotong University, Shanghai 200240, China.
- Publication Type:Journal Article
- MeSH:
Bioreactors;
Cell Culture Techniques;
Gametogenesis;
physiology;
Germ Cells;
physiology;
Laminaria;
growth & development;
radiation effects;
Light;
Shear Strength;
Stress, Mechanical
- From:
Chinese Journal of Biotechnology
2007;23(5):935-940
- CountryChina
- Language:Chinese
-
Abstract:
Laminaria japonica gametophytic cells were cultivated in a photobioreactor under continuous shear stress (0-1000 r/min) in 60 hours and the following static cultivation within 23.5 days. The content of chlorophyll a reached the maximum value of 2.36 mg/L at the end of continuous shear stress when the agitation speed was 90 r/min, while the chlorophyll a (chl a) concentration decreased quickly and nitrogen and phosphorus were released under high shear force (270-1000 r/min). The cell injury ratio at 1000r/min was as 18 times as that of the control. During the recovery course, gametophytic cells showed themselves distinct recovery capability at all agitation speeds. Furthermore, the content of chl a is a more exact index as biomass than dry cells weight (DCW). Besides cell injury ratio, the liberation of phosphorus demonstrates the cells injury.
