Effect of fatty acids from plastrum testudinis on proliferation of rat bone mesenchymal stem cell.
- Author:
Yue-Hua ZHANG
1
;
He-Ping ZENG
;
Dong-Feng CHEN
Author Information
1. Guangzhou City Polytechnic College, Guangzhou 510405, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Bone Marrow Cells;
cytology;
Cell Proliferation;
drug effects;
Cells, Cultured;
Fatty Acids;
isolation & purification;
pharmacology;
Materia Medica;
chemistry;
pharmacology;
Mesenchymal Stromal Cells;
cytology;
Rats;
Turtles
- From:
Chinese Journal of Biotechnology
2007;23(6):1029-1032
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the components in Plastrum Testudinis which have effects on the proliferation of rat bone marrow mesenchymal stem cells( bMMSCs), the active parts of plastrum testudinis which can promote proliferation of rat mesenchymal stem cells were extracted by petroleum aether. The activities of inducing the proliferation of bMMSCs were studied by MTT assay and flow cytometry. The chemical components of extraction were analyzed by GC-MS. The results showed that the petroleum aether extraction can obviously promote the proliferation of the stem cells. The main components are long-chain fatty acids, cholesterols and cholest-4-en-3-one, and palmitic acid, stearic acid and cholest-4-en-3-one have effects on proliferation of bMMSCs. In plastrum testudinis, fatty acids can promote the proliferation of bMMSCs but not increase overly. This provide the experiment basis, and offer important reference for Traditional Chinese Medicine that researches stem cells.
