Kinetic model for exopolysaccharides (EPS) of Ganoderma lucidum in batch fermentation.
- Author:
Jian-Guo ZHANG
1
;
Xiao-Ming CHEN
;
Xin-Sheng HE
Author Information
1. School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China. zjg730328@126.com
- Publication Type:Journal Article
- MeSH:
Culture Techniques;
methods;
Extracellular Space;
metabolism;
Fermentation;
Kinetics;
Models, Biological;
Polysaccharides;
biosynthesis;
Reishi;
chemistry;
growth & development
- From:
Chinese Journal of Biotechnology
2007;23(6):1065-1070
- CountryChina
- Language:Chinese
-
Abstract:
The synthetic characteristic of extracellular polysaccharide (EPS) of Ganoderma lucidum performed in batch fermentation was studied. The result showed that the production EPS was partially growth-associated. The cell dry weight (CDW) and EPS reached 15.56 g x L(-1), 3.02 g x L(-1) respectively. The yield of EPS to cell dry weight ( Y(p/x)) was 0.19. Based on the test result of batch fermentation, a kinetic model was proposed by using the Logistic equation for cell growth, the Luedeking Piret equation for EPS production and the Luedeking-piret-like equation for consumption of glucose as substrate. The calculated results of models were compared satisfactorily with experimental data under various glucose concentrations, the average relative error was no more than 5%. The kinetic model had practically guiding producing PES in fermentation of Ganoderma lucidum.