Cloning and sequence analysis of SOCS-2 gene in pig.
- Author:
Bao-Wen DU
1
;
Gong-She YANG
;
Chao SUN
Author Information
1. Northwest A&F University, Laboratory of Animal Fat Deposition and Muscle Development, Yang Ling 712100, China.
- Publication Type:Journal Article
- MeSH:
Amino Acid Sequence;
Animals;
Base Sequence;
Cloning, Molecular;
DNA, Complementary;
Humans;
Molecular Sequence Data;
Protein Structure, Secondary;
genetics;
Rats;
Reverse Transcriptase Polymerase Chain Reaction;
Sequence Analysis;
Sequence Homology;
Suppressor of Cytokine Signaling Proteins;
genetics;
Swine;
genetics
- From:
Chinese Journal of Biotechnology
2007;23(6):1091-1096
- CountryChina
- Language:Chinese
-
Abstract:
Total RNA was isolated from kidney of BaMei pig, a local strain of Chinese pig, and then the cDNA sequence of SOCS-2 gene was cloned by RT-PCR (GenBank accepted number is EF121242). Then the cloned SOCS-2 gene was inserted into PMD19-T vector by T/A cloning, transformed into DH-5alpha, tested by PCR and sequenced. The data show that the homology of the cloned porcine SOCS-2, including 822 bp, is more than 93% and that of the deduced amino acid sequence is 89% when compared with human, rat and mice. And the molecular weight of SOCS-2 protein is about 22.25 kD and PI is 8.03. The cloning of SOCS-2 gene is useful for the further research on the molecular mechanism by which regulating growth and development of organism.
