The expression, purification and activity analysis of the rice chitinase gene in Escherichia coli.
- Author:
Aikui CHEN
1
;
Lujun YU
;
Jianming FAN
;
Dongru FENG
;
Jinfa WANG
Author Information
1. Biology Department, Guangdong Institute of Education, Guangzhou 510303, China.
- Publication Type:Journal Article
- MeSH:
Chitin;
metabolism;
Chitinases;
biosynthesis;
genetics;
Cloning, Molecular;
Escherichia coli;
genetics;
metabolism;
Molecular Sequence Data;
Oryza;
enzymology;
genetics;
Recombinant Proteins;
biosynthesis;
genetics;
isolation & purification;
metabolism
- From:
Chinese Journal of Biotechnology
2008;24(2):188-192
- CountryChina
- Language:Chinese
-
Abstract:
The cloned cDNA sequence of rice (Oryza sativa L. Cpslo17) chitinase gene Oschi was cloned, (which was registered in GenBank, the accession number: EU045451) ligated with the expression vector pGEX-4T-1, and transformed into E. coli BL21(DE3). The expression of Oschi was induced by IPTG, and the conditions were optimized. After purification the in vitro activity of Oschi chitinase was analyzed, and the results indicated that it could efficiently degrade chitin.
