Selection and identification of salt-tolerant variants of Taraxacum officinale.
- Author:
Xinguo ZHANG
1
;
Yinxin LI
;
Hua CHEN
;
Wuliang SHI
Author Information
1. Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
- Publication Type:Journal Article
- MeSH:
Culture Techniques;
methods;
Drug Tolerance;
genetics;
Flavones;
analysis;
Genetic Variation;
genetics;
Plant Leaves;
genetics;
growth & development;
Random Amplified Polymorphic DNA Technique;
Salt-Tolerant Plants;
genetics;
growth & development;
Seedlings;
genetics;
growth & development;
Sodium Chloride;
pharmacology;
Superoxide Dismutase;
analysis;
Taraxacum;
genetics;
growth & development
- From:
Chinese Journal of Biotechnology
2008;24(2):262-271
- CountryChina
- Language:Chinese
-
Abstract:
In order to obtain salt-tolerant variant plants of Dandelion (Taraxacum officinale Weber), the leaf discs were excised from 20 to 30-day old seedlings to produce callus, then the induced calli were transferred to selection mediums containing 1.5% NaCl. After regenerating and rooting, these salt-tolerant calli finally developed into 12 variant plantlets. Compared with the wild-type, these regenerated plants produced more trichomes on their leaves, and had larger leaves and shorter petioles. Additionally, the dumpy roots and an approximately 2-cm bract in middle parts of the floricanes were clearly observed in these salt-tolerant plants. By RAPD (Random Amplified Polymorphic DNA) and SDS-PAGE analysis, these salt-tolerant plants showed differences from the control at DNA and protein levels. With 1.5% NaCl treatment, the antioxidant enzyme activity, proline content, and flavonoid concentration were higher in these salt-tolerant plants, whereas maloaldehyde concentration was significantly lower. Salt-tolerant lines of T. officinale showed stronger anti-oxidative activity and higher flavonoid contents.
