Expression of a testis-specific gene 1700001022RIK in mice and its bioinformatic analysis.
- Author:
Yu-chi LI
;
Shou-ren LIN
;
Man-ling LUO
;
Huan GUO
;
Han-wei WU
;
Zhi-mao JIANG
;
Yao-ting GUI
- Publication Type:Journal Article
- MeSH: Age Factors; Animals; Blotting, Western; Computational Biology; DNA, Complementary; Gene Expression; Genomics; Male; Mice; Molecular Chaperones; genetics; Seminiferous Tubules; Spermatids; Spermatocytes; Spermatogenesis; genetics; Spermatogonia; Testis
- From: National Journal of Andrology 2015;21(5):391-395
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify the expression characteristics of the 1700001022RIK (RIKEN cDNA 1700001022) gene in mice and explore its function by bioinformatic analysis.
METHODSUsing the expression profile of gene microarray, we detected the expression of a new testis-specific gene, 1700001022RIK, in mice. We analyzed its expression characteristics in the testis tissue and their changes in different developmental stages of the testis by RT-PCR, real-time RT-PCR, Western blot, and immunohistochemistry. We performed bioinformatic analysis using a bioinformatic software.
RESULTSThe 1700001022RIK gene was specifically expressed in the mouse testis in an age-dependent manner, most highly in the adult mice. The 1700001022RIK protein was mainly expressed in the spermatogonia, spermatocytes, and round spermatids of the adult mice. Bioinformatic analysis showed that the 1700001022RIK protein amino acid sequence had a high similarity in human and mice, which indicated that this gene was highly conserved in mammals.
CONCLUSION1700001022RIK is a testis-specific gene mainly expressed in the spermatogonia, spermatocytes, and round spermatids of seminiferous tubules, which might be involved in the regulation of spermatogenesis.