Lipopolysaccharide, TNFα, IL-6, dexamethasone, and insulin increase the expression of GPR54 in the MCF7 breasr cancer cell line.
- Author:
Bing-kun HUANG
;
Jiang-feng MAO
;
Zhao SUN
;
Qin HAN
;
Min NIE
;
Xue-yan WU
- Publication Type:Journal Article
- MeSH: Blotting, Western; Dexamethasone; administration & dosage; pharmacology; Glucocorticoids; administration & dosage; pharmacology; Gonads; drug effects; metabolism; Humans; Hypoglycemic Agents; administration & dosage; pharmacology; Insulin; administration & dosage; pharmacology; Interleukin-6; administration & dosage; pharmacology; Lipopolysaccharides; administration & dosage; pharmacology; MCF-7 Cells; RNA, Messenger; metabolism; Real-Time Polymerase Chain Reaction; Receptors, G-Protein-Coupled; drug effects; genetics; metabolism; Receptors, Kisspeptin-1; Time Factors; Tumor Necrosis Factor-alpha; administration & dosage; pharmacology
- From: National Journal of Andrology 2015;21(7):587-592
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of different concentrations of lipopolysaccharide (LPS), tumor necrosis factor α (TNFα), interleukin-6 (IL-6), dexamethasone (Dex), and insulin on the mRNA and protein expressions of GPR54 in the MCF7 cell line in vitro.
METHODSMCF7 breasr cancer cells were cultured and treated with different concentrations of LPS (10 and 20 µg/ml), TNFα (20 and 100 ng/ml), IL-6 (10 and 20 ng/ml), Dex (10(-6) and 10(-7) mol/L), and insulin (0.01 and 0.1 IU/L). Those treated with culture fluid only served as controls. The mRNA and protein expressions of GPR54 were measured by real-time PCR and Western blot, respectively, after 6, 24, 48, and 72 hours of treatment.
RESULTSCompared with the blank con- trol, LPS (10 and 20 µg/ml), TNFα (20 and 100 ng/ml), IL-6 (10 and 20 ng/ml), Dex (10(-6) and 10(-7) mol/L), and insulin (0.01 and 0.1 IU/L) significantly increased the expressions of GPR54 mRNA (P < 0.05) and protein (P < 0.05).
CONCLUSIONLPS, TNFα, IL-6, Dex, and insulin evidently increase the expression of GPR54 in the MCF7 cell line, indicating their influence on the function of gonads by regulating the GPR54 level.