Platelet-derived growth factor-BB induces phenotypic transformation of corpus cavernosum smooth muscle cells in SD rats.
- Author:
Jin-tai LUO
;
Wen-jun YU
;
An-yang WEI
;
Guo-hua ZENG
- Publication Type:Journal Article
- MeSH: Actins; metabolism; Animals; Calcium-Binding Proteins; metabolism; Cell Culture Techniques; Cells, Cultured; Male; Microfilament Proteins; metabolism; Muscle Contraction; Myocytes, Smooth Muscle; cytology; drug effects; metabolism; Myosin Heavy Chains; metabolism; Penis; cytology; drug effects; metabolism; Phenotype; Proto-Oncogene Proteins c-sis; administration & dosage; pharmacology; RNA, Messenger; metabolism; Rats; Rats, Sprague-Dawley; Time Factors
- From: National Journal of Andrology 2015;21(7):593-597
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the effect of the platelet-derived growth factor-BB (PDGF-BB) on the phenotypic transformation of corpus cavernosum smooth muscle cells (CCSMC) in SD rats.
METHODSCCSMCs were primarily cultured in the modified tissue sticking medium and subjected to immunofluorescence assay. The cells were divided into a blank control and four PDGF-BB groups, the latter exposed to 5, 10, 20, and 40 ng/ml of PDGF-BB, respectively, for 24 hours, and the cells in the 20 ng/ml PDGF-BB group treated for 24, 48, and 72 hours. The the relative expressions of α-SMA, SMMHC, calponin, and OPN mRNA were determined by real-time fluorescence quantitative RT-PCR (qRT-PCR).
RESULTSThe α-SMA positive rate of the CCSMCs was over 95%. Compared with the blank control group, the expression levels of α-SMA, SMMHC, and calponin mRNA were significantly decreased (P < 0.05) while that of OPN mRNA remarkably increased (P < 0.05) in the PDGF-BB groups. The 20 ng/ml PDGF-BB group also showed significantly downregulated expressions of α-SMA, SMMHC, and calponin mRNA (P < 0.05) and upregulated expression of OPN mRNA (P < 0.05) at 24, 48, and 72 hours.
CONCLUSIONPDGF-BB can induce the transformation of the phenotype of CCSMCs in SD rats from the contractile to the synthetic type.