An optimal method for cryopreservation of microamount round spermatids of the mouse.
- Author:
Hong JIANG
;
Li WANG
;
Cun-li WANG
- Publication Type:Journal Article
- MeSH: Animals; Cell Survival; Cryopreservation; methods; Cryoprotective Agents; administration & dosage; pharmacology; Glycerol; administration & dosage; pharmacology; Male; Mice; Spermatids; Time Factors; Vitrification; drug effects
- From: National Journal of Andrology 2015;21(8):698-701
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo search for an optimal protocol and freezing conditions for the cryopreservation of microamount round spermatids of the mouse.
METHODSWe compared the survival rates of frozen-thawed microamount round spermatids of the mouse achieved by vitrification or standard slow freezing with different concentrations of glycerol (5, 7, or 9%) and different lengths of equilibrium time (0, 15, 30, 45, or 60 min).
RESULTSUnder the conditions of 7% glycerol and 30 min equilibrium, both vitrification and standard slow freezing achieved high survival rates of spermatids, and the former obtained an even higher rate than the latter ([72.9 ± 15.4]% vs [58.2 ± 17.7]%, P < 0.05).
CONCLUSIONA high rate of frozen-thawed microamount round spermatids of the mouse can be achieved by vitrification under the conditions of 7% glycerol and 30 min equilibrium.